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KMID : 0359319940340020327
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Korean Journal of Veterinary Research
1994 Volume.34 No. 2 p.327 ~ p.333
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Identification of Aujeszky´s disease virus by in situ hybridization
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Abstract
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The purpose of this study was to establish a rapid diagnostic method detecting Aujeszky´s disease virus (ADV) DNA in the cultured cell monolayers (PK-15) and tissue sections of ADV(NYJ-I-87)-infected rats and pigs by in situ hybridization(ISH).
Detection of specific ADV-DNA in infected cells was conducted by radiolabeled ISH method using "P-labeled DNA probe (BamHl 7 fragment) which contains a 6.3 Kb ADV-DNA insert.
Where ADV-DNA was detected by radiolabeled ISH, the deposition of black photographic grains occurred in the nuclei and the cytoplasms of ADV-infected cells. Positive hybridization signal was often observed in the spinal trigerminal nucleus of the pons, the nucleus of the trigerminal ganglion neuron and the epithelial cells of tonsillar crypts. The results suggested that ISH is considered as a highly sensitive and reliable tool for confirmative diagnosis of this viral disease.
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